Anti-ATP5D Antibody (CAB9929)

Antibody Name:	Anti-ATP5D Antibody
Antibody SKU:	CAB9929
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human ATP5D (NP_001678.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Raji, HepG2, A-549, Mouse kidney, Mouse heart, Mouse liver, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human ATP5D (NP_001678.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLPA ALLR RPGL GRLV RHAR AYAE AAAA PAAA SGPN QMSF TFAS PTQV FFNG ANVR QVDV PTLT GAFG ILAA HVPT LQVL RPGL VVVH AEDG TTSK YFVS SGSI AVNA DSSV QLLA EEAV TLDM LDLG AAKA NLEK AQAE LVGT ADEA TRAE IQIR IEAN EALV KALE
Gene ID:	513
Uniprot:	P30049
Cellular Location:	Mitochondrion, Mitochondrion inner membrane
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

ATP5D

Background:

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.

UniProt Protein Function:	ATP5D: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase epsilon chain family.
UniProt Protein Details:	Protein type:EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase; Mitochondrial Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity Biological Process: ATP biosynthetic process; ATP synthesis coupled proton transport; mitochondrial ATP synthesis coupled proton transport
NCBI Summary:	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P30049
NCBI GenInfo Identifier:	584812
NCBI Gene ID:	513
NCBI Accession:	P30049.2
UniProt Secondary Accession:	P30049,Q6FG90, D6W5Y3,
UniProt Related Accession:	P30049
Molecular Weight:	17kDa
NCBI Full Name:	ATP synthase subunit delta, mitochondrial
NCBI Synonym Full Names:	ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
NCBI Official Symbol:	ATP5D
NCBI Protein Information:	ATP synthase subunit delta, mitochondrial
UniProt Protein Name:	ATP synthase subunit delta, mitochondrial
UniProt Synonym Protein Names:	F-ATPase delta subunit
UniProt Gene Name:	ATP5D

	Western blot analysis of extracts of various cell lines, using ATP5D antibody (CAB9929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using ATP5D antibody (CAB9929) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using ATP5D antibody (CAB9929) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using ATP5D antibody (CAB9929) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ATP5D Antibody (PACO04531)

ATP5D Antibody (PACO07924)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)