Anti-ATP5C1 Antibody (CAB15257)

Antibody Name:	Anti-ATP5C1 Antibody
Antibody SKU:	CAB15257
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, U-87MG, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ATLK DITR RLKS IKNI QKIT KSMK MVAA AKYA RAER ELKP ARIY GLGS LALY EKAD IKGP EDKK KHLL IGVS SDRG LCGA IHSS IAKQ MKSE VATL TAAG KEVM LVGI GDKI RGIL YRTH SDQF LVAF KEVG RKPP TFGD ASVI ALEL LNSG YEFD EGSI IFNK FRSV ISYK TEEK PIFS LNTV ASAD SMSI YDDI DADV LQNY QEYN LANI IYYS LKES TTSE QSAR MTAM DNAS KNAS EMID KLTL TFNR TRQA VITK ELIE IISG AAAL D
Gene ID:	509
Uniprot:	P36542
Cellular Location:	Mitochondrion, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	32kDa
Observed MW:	36kDa

Synonyms:

ATP5C1, ATP5C, ATP5CL1

Background:

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

UniProt Protein Function:	ATP5C: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase gamma chain family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Energy Metabolism - oxidative phosphorylation; Mitochondrial; Hydrolase; EC 3.6.3.14 Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; myelin sheath Molecular Function:ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; transmembrane transporter activity Biological Process: ATP biosynthetic process; cellular metabolic process; mitochondrial ATP synthesis coupled proton transport; oxidative phosphorylation
NCBI Summary:	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq, Jul 2008]
UniProt Code:	P36542
NCBI GenInfo Identifier:	543875
NCBI Gene ID:	509
NCBI Accession:	P36542.1
UniProt Secondary Accession:	P36542,Q5VYP3, Q6I9V2, Q96AS8, A8KA31,
UniProt Related Accession:	P36542
Molecular Weight:	32,881 Da
NCBI Full Name:	ATP synthase subunit gamma, mitochondrial
NCBI Synonym Full Names:	ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
NCBI Official Symbol:	ATP5C1
NCBI Official Synonym Symbols:	ATP5C; ATP5CL1
NCBI Protein Information:	ATP synthase subunit gamma, mitochondrial
UniProt Protein Name:	ATP synthase subunit gamma, mitochondrial
UniProt Synonym Protein Names:	F-ATPase gamma subunit
UniProt Gene Name:	ATP5C1
UniProt Entry Name:	ATPG_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (CAB15257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5S.
	Immunohistochemistry of paraffin-embedded rat ovary using ATP5C1 antibody (CAB15257) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using ATP5C1 antibody (CAB15257) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using ATP5C1 antibody (CAB15257) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ATP5C1 Polyclonal Antibody (CAB15257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

ATP5C1 Antibody (PACO00440)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)