The ATP2C2 Polyclonal Antibody (PAC06239) is a valuable tool for researchers studying ATP2C2, a crucial protein involved in calcium transport and homeostasis within cells. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ATP2C2 protein, this antibody allows for the detection and analysis of ATP2C2 expression in various cell types, making it ideal for research in cell biology and calcium signaling pathways.ATP2C2, also known as secretory pathway calcium ATPase 2, plays a vital role in maintaining calcium levels within organelles such as the Golgi apparatus, contributing to proper cell function and signaling.
Dysregulation of ATP2C2 activity has been linked to various diseases, making it a promising target for therapeutic interventions. Understanding the function of ATP2C2 is essential for unraveling its role in health and disease, and this antibody provides a valuable tool for further exploration in these areas.
Antibody Name:
ATP2C2 Antibody (PACO62239)
Antibody SKU:
PACO62239
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Calcium-transporting ATPase type 2C member 2 protein (621-702AA)
Immunofluorescence staining of A549 cells with PACO62239 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.
Synonyms:
Calcium-transporting ATPase type 2C member 2 (ATPase 2C2) (EC 3.6.3.8) (Secretory pathway Ca(2+)-ATPase 2), ATP2C2, KIAA0703 SPCA2
UniProt Protein Function:
ATP2C2: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.6.3.8; Membrane protein, integral; Hydrolase; Transporter; Transporter, ion channel; Membrane protein, multi-passChromosomal Location of Human Ortholog: 16q24.1Cellular Component: Golgi membrane; integral to membraneMolecular Function: protein binding; calcium-transporting ATPase activity; metal ion binding; ATP bindingBiological Process: metabolic process; transmembrane transport
