The ATP2B4 Polyclonal Antibody (CAB10105) is specifically designed for research involving ATP2B4, a crucial plasma membrane calcium ATPase that plays a key role in calcium homeostasis in cells. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the ATP2B4 protein, it enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and physiology.
ATP2B4, also known as PMCA4, is essential for maintaining intracellular calcium levels and regulating calcium signaling pathways in cells. Dysregulation of ATP2B4 activity has been implicated in various diseases, including cardiovascular disorders, neurodegenerative diseases, and cancer. Research on ATP2B4 is crucial for understanding the mechanisms underlying these conditions and could potentially lead to the development of novel therapeutic interventions targeting calcium dysregulation.
Product Name:
ATP2B4 Rabbit Polyclonal Antibody
SKU:
CAB10105
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1205 of human ATP2B4 (NP_001675.3).
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
493
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse brain, using ATP2B4 antibody (CAB10105) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
