The ATP2B3 Polyclonal Antibody (PAC062447) is a powerful tool for studying ATP2B3, a crucial protein involved in cellular calcium transport and homeostasis. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications.ATP2B3 is a key player in maintaining intracellular calcium levels and regulating various cellular processes, including muscle contraction, neurotransmitter release, and gene expression. Dysregulation of ATP2B3 has been linked to a range of diseases, such as heart failure, neurological disorders, and cancer.
By targeting ATP2B3, researchers can gain deeper insights into cellular calcium dynamics and develop novel therapeutic strategies for treating related pathologies.With its ability to recognize and bind to ATP2B3, this antibody enables precise detection and analysis of the protein in different cell types and tissues. Its reliability and versatility make it an invaluable tool for investigating the role of ATP2B3 in health and disease, paving the way for advancements in molecular biology, physiology, and drug development.
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: ATP2B3 antibody at 1:500. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 135, 129, 128, 133, 127, 125, 123 kDa. Observed band size: 135 kDa.
Immunofluorescence staining of Hela cells with PACO62447 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
ATP2B3: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Transporter; Hydrolase; Membrane protein, multi-pass; Transporter, ion channel; EC 3.6.3.8Chromosomal Location of Human Ortholog: Xq28Cellular Component: Golgi apparatus; integral to plasma membrane; intracellular membrane-bound organelle; plasma membraneMolecular Function: calcium-transporting ATPase activity; PDZ domain bindingBiological Process: cellular calcium ion homeostasis; transportDisease: Spinocerebellar Ataxia, X-linked 1
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
