The ATP2B1 Polyclonal Antibody (CAB18688) is a valuable tool for researchers studying ATP2B1, a protein responsible for calcium transportation in cells. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ATP2B1 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in cell biology and physiological research.ATP2B1, also known as Plasma Membrane Calcium-Transporting ATPase 1, plays a crucial role in maintaining calcium homeostasis within cells, which is essential for numerous cellular processes including muscle contraction, neurotransmission, and cell signaling.
Dysregulation of ATP2B1 has been implicated in various diseases such as cardiovascular disorders, neurological conditions, and cancer. Investigating the function of ATP2B1 is vital for understanding the mechanisms underlying these diseases and developing targeted therapies to combat them.
Product Name:
ATP2B1 Rabbit Polyclonal Antibody
SKU:
CAB18688
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ATP2B1 (NP_001673.2).
apical plasma membrane, basolateral plasma membrane, cytoplasmic side of plasma membrane, extracellular exosome, GABA-ergic synapse, glutamatergic synapse, immunological synapse, nucleoplasm, photoreceptor ribbon synapse, plasma membrane, presynaptic active zone membrane, presynaptic membrane, synaptic vesicle membrane
Calculated MW:
135kDa
Observed MW:
140kDa
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
490
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse brain, using ATP2B1 antibody (CAB18688) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
