Anti-ATP2A2/SERCA2 Antibody (CAB1097)

Antibody Name:	Anti-ATP2A2 / SERCA2 Antibody
Antibody SKU:	CAB1097
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human ATP2A2 / SERCA2 (NP_733765.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, THP-1, PC-3, MCF7, A673, HeLa, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human ATP2A2 / SERCA2 (NP_733765.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NAEN AIEA LKEY EPEM GKVY RQDR KSVQ RIKA KDIV PGDI VEIA VGDK VPAD IRLT SIKS TTLR VDQS ILTG ESVS VIKH TDPV PDPR AVNQ DKKN MLFS GTNI AAGK AMGV VVAT GVNT EIGK IRDE MVAT EQER TPLQ QKL
Gene ID:	488
Uniprot:	P16615
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:	109kDa/111kDa/114kDa
Observed MW:	115kDa

Synonyms:

ATP2B, DAR, DD, SERCA2, ATP2A2/SERCA2, ATP2A2

Background:

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transporter; Transporter, ion channel; EC 3.6.3.8; Endoplasmic reticulum; Cell adhesion; Hydrolase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane; membrane; sarcoplasmic reticulum; sarcoplasmic reticulum membrane Molecular Function:calcium ion binding; calcium-transporting ATPase activity; enzyme binding; protein binding Biological Process: cell adhesion; elevation of endoplasmic reticulum calcium ion concentration; endoplasmic reticulum calcium ion homeostasis; epidermis development; positive regulation of heart rate Disease: Acrokeratosis Verruciformis; Darier-white Disease
NCBI Summary:	This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]
UniProt Code:	P16615
NCBI GenInfo Identifier:	114312
NCBI Gene ID:	488
NCBI Accession:	P16615.1
UniProt Secondary Accession:	P16615,P16614, Q86VJ2, A6NDN7, B4DF05,
UniProt Related Accession:	P16615
Molecular Weight:	109,734 Da
NCBI Full Name:	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
NCBI Synonym Full Names:	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
NCBI Official Symbol:	ATP2A2
NCBI Official Synonym Symbols:	DD; DAR; ATP2B; SERCA2
NCBI Protein Information:	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Protein Name:	Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
UniProt Synonym Protein Names:	Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Protein Family:	Sarcoplasmic/endoplasmic reticulum calcium ATPase
UniProt Gene Name:	ATP2A2
UniProt Entry Name:	AT2A2_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP2A2 / SERCA2 antibody (CAB1097) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using ATP2A2 / SERCA2 Antibody (CAB1097) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ATP2A2 / SERCA2 Antibody (CAB1097) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ATP2A2 / SERCA2 antibody (CAB1097). Blue: DAPI for nuclear staining.

Antibodies

Anti-ATP2A2 / SERCA2 Antibody (CAB0098)

Anti-SERCA2 Antibody (CAB11692)

Anti-Phospho-ATP2A2-Thr484 Antibody (CABP1070)

ATP2A2 Antibody (PACO02127)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)