The ATP2A2 Polyclonal Antibody (CAB0098) is a valuable tool for researchers studying ATP2A2, a protein involved in regulating calcium levels in cells. This antibody, raised in rabbits, is highly specific to ATP2A2 in human samples and is validated for use in Western blot applications. By binding to the ATP2A2 protein, researchers can easily detect and analyze its expression in various cell types, making it ideal for investigations in cell biology and calcium signaling pathways.ATP2A2, also known as SERCA2, plays a crucial role in maintaining cellular calcium homeostasis, which is essential for cell function and muscle contraction.
Dysregulation of ATP2A2 has been implicated in various diseases, including heart failure, muscular disorders, and neurodegenerative conditions. By studying ATP2A2 expression and activity, researchers can gain insights into the pathophysiology of these diseases and identify potential therapeutic targets to restore calcium balance in affected cells. Overall, the ATP2A2 Polyclonal Antibody is a valuable tool for researchers interested in studying the role of ATP2A2 in cellular function and disease pathways, offering precise and reliable detection of ATP2A2 protein levels in experimental settings.
Product Name:
SERCA2/ATP2A2 Rabbit Polyclonal Antibody
SKU:
CAB0098
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human SERCA2/ATP2A2 (NP_733765.1).
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Other types of mutations in this gene have been associated with various forms of muscular dystrophies. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
488
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using SERCA2/ATP2A2 Rabbit pAb (CAB0098) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
