ATP Assay Kit (Luminescent) (BA0080)

SKU:

BA0080

Product Type:

Assay

Detection Method:

Luminometric

Sample Type:

Cells

Research Area:

Glycolysis & Carbohydrates

TCA Cycle

Kidney Biomarker

Description

Description

ATP Assay Kit - Information

Assay Genies ATP Assay Kit (Luminescent) provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates

Applications

For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.

ATP Assay Kit - Key Features

Safe. Non-radioactive assay.
Sensitive and accurate. As low as 0.02 uM ATP or a single cell can be quantified.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS: Z' factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

ATP Assay Kit- Data Sheet
Kit Includes	Assay Buffer: 10 mL Substrate: 120 mL ATP Enzyme: 120 mL Standard: 100 mL 3 mM ATP
Method of Detection	Luminescence
Detection Limit	0.1 uM
Samples	Cells etc
Species	All
Protocol Length	10 min
Size	100 tests
Storage	Store all reagents at -20°C.
Shelf Life	12 months after receipt.

More Details

Adenosine 5'-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.