The ATOH8 Polyclonal Antibody (CAB17232) is a research tool designed for studying the ATOH8 protein, which plays a key role in the regulation of gene expression during development. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in various applications, including Western blotting.ATOH8, a transcription factor belonging to the basic helix-loop-helix (bHLH) family, is known to be involved in processes such as neurogenesis and cell differentiation. By specifically binding to ATOH8, this polyclonal antibody enables scientists to detect and study the function of this protein in different cell types, making it a valuable tool for research in developmental biology and related fields.
The role of ATOH8 in regulating gene expression and cell fate determination makes it a promising target for investigating various developmental processes and diseases. Understanding the function of ATOH8 and its regulatory mechanisms could provide insights into potential therapeutic strategies for conditions related to abnormal cell differentiation and tissue development.
Product Name:
ATOH8 Rabbit Polyclonal Antibody
SKU:
CAB17232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ATOH8 (NP_116216.2).
Sequence:
LRPR ILLC APPA RPAP SAPP APPA PPES TVRP APPT RPGE SSYS SISH VIYN NHQD SSAS PRKR PGEA TAAS SEIK ALQQ TRRL LANA RERT RVHT ISAA F
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HATH6; bHLHa21; ATOH8
Positive Sample:
Mouse brain,Mouse kidney
Cellular Localization:
cytoplasm, nuclear speck, nucleoplasm, nucleus
Calculated MW:
35kDa
Observed MW:
35kDa
Enables DNA-binding transcription factor activity and E-box binding activity. Involved in several processes, including SMAD protein signal transduction; positive regulation of endothelial cell differentiation; and regulation of gene expression. Located in nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
84913
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATOH8 Rabbit pAb (CAB17232) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
