The ATM Polyclonal Antibody (CAB5908) is a valuable tool for researchers studying the ATM protein, a key player in DNA damage response and repair pathways. This antibody, produced in rabbits, offers high reactivity with human samples and is validated for use in various applications such as Western blot and immunofluorescence.ATM, or Ataxia Telangiectasia Mutated, is a crucial protein involved in sensing DNA damage and activating cell cycle checkpoints and DNA repair mechanisms. Dysregulation of ATM function has been implicated in various diseases, including cancer and neurodegenerative disorders.
By targeting ATM with this polyclonal antibody, researchers can gain insights into its role in maintaining genomic stability and its potential as a therapeutic target for diseases characterized by DNA damage.With its versatility and specificity, the ATM Polyclonal Antibody (CAB5908) is an essential tool for advancing research in the fields of genomics, oncology, and molecular biology. Its ability to detect and analyze ATM protein expression in different cell types makes it an invaluable asset for investigating the intricate mechanisms of DNA damage response and repair pathways.
Product Name:
ATM Rabbit Polyclonal Antibody
SKU:
CAB5908
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human ATM (NP_000042.3).
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
Purification Method:
Affinity purification
Gene ID:
472
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of H460 cells, using ATM Antibody (CAB5908) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
