The ATIC Polyclonal Antibody (CAB5559) is a valuable tool for researchers studying ATIC, a key enzyme involved in the de novo purine biosynthetic pathway. This antibody, raised in rabbits, has high reactivity with human samples and is suitable for use in Western blot applications. By binding to the ATIC protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in biochemistry, molecular biology, and cancer research.ATIC plays a crucial role in nucleotide metabolism, making it a potential target for cancer therapy and other diseases characterized by dysregulated cellular proliferation.
By understanding the function and regulation of ATIC, researchers can gain insights into the mechanisms of nucleotide biosynthesis and identify potential therapeutic strategies for targeting this enzyme in cancer cells.Overall, the ATIC Polyclonal Antibody (CAB5559) serves as a valuable tool for investigating the role of ATIC in cellular metabolism and disease pathogenesis, offering researchers a reliable and versatile option for their experimental needs.
Product Name:
ATIC Rabbit Polyclonal Antibody
SKU:
CAB5559
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ATIC (NP_004035.2).
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.
Purification Method:
Affinity purification
Gene ID:
471
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATIC antibody (CAB5559) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
