The ATG8 Polyclonal Antibody is a valuable tool for researchers studying autophagy, a cellular process essential for maintaining cellular homeostasis and responding to stress. This antibody, raised in rabbits, is highly specific for ATG8 protein and is validated for use in various applications, including Western blot and immunofluorescence.ATG8 is a key protein involved in autophagy, playing a crucial role in the formation of autophagosomes and the degradation of cellular components.
Dysregulation of ATG8 has been implicated in various diseases, including neurodegenerative disorders and cancer. By targeting ATG8 with this antibody, researchers can better understand the mechanisms of autophagy and its implications in disease pathology.Overall, the ATG8 Polyclonal Antibody is an essential tool for studying autophagy and its role in disease, providing valuable insights into potential therapeutic strategies targeting this pathway.
Product Name:
ATG8 Polyclonal Antibody
SKU:
CAB22294
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana,Zea mays,Triticum aestivum,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequenceof Triticum aestivum ATG8.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
Arabidopsis thaliana,Zea mays,Triticum aestivum
Conjugate:
Unconjugated
Cellular Localization:
autophagosome
Calculated MW:
14kDa
Observed MW:
15kDa
nan
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Zea mays, using ATG8 Rabbit pAb (CAB22294) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.