The ATG7 Rabbit Monoclonal Antibody (CAB19604) is a powerful tool for researchers studying autophagy, a crucial cellular process involved in maintaining cellular homeostasis and responding to stress. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards ATG7 protein in human samples.ATG7 is a key component of the autophagy pathway, responsible for the conjugation of ATG8/LC3 to phosphatidylethanolamine and promoting the formation of autophagosomes. Dysregulation of autophagy has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic conditions, making ATG7 an important target for therapeutic interventions.
This antibody is validated for use in Western blot applications, enabling researchers to detect and analyze ATG7 expression in different cell types and tissues. By studying the role of ATG7 in autophagy, researchers can gain insights into the underlying mechanisms of disease pathology and potentially identify novel therapeutic strategies targeting autophagy.
Product Name:
ATG7 Rabbit Monoclonal Antibody
SKU:
CAB19604
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atg7(Apg7) (O95352).
This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10533
Clone Number:
ARC0083
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] ATG7 Rabbit mAb (CAB19604) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
