ATG4D Polyclonal Antibody (CAB21690)
- SKU:
- CAB21690
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IF
Description
| Product Name: | ATG4D Polyclonal Antibody |
| SKU: | CAB21690 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3). |
| Sequence: | MNSV SPAA AQYR SSSP EDAR RRPE ARRP RGPR GPDP NGLG PSGA SGPA LGSP GAGP SEPD EVDK FKAK FLTA WNNV KYGW VVKS RTSF SKIS SIHL CGRR YRFE GEGD IQRF QRDF VSRL WLTY RRDF PPLP GGCL TSDC GWGC MLRS GQMM LAQG LLLH FLPR DWTW AEGM GLGP PELS GSAS PSRY HGPA RWMP PRWA QGAP ELEQ ERRH RQIV SWFA DHPR APFG LH |
| Tested Applications: | WB IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
| Synonyms: | APG4D; AUTL4; APG4-D; HsAPG4D; ATG4D |
| Positive Sample: | 22Rv1,NCI-H460,Mouse heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Mitochondrion matrix. |
| Calculated MW: | 53kDa |
| Observed MW: | Refer to figures |
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
| Purification Method: | Affinity purification |
| Gene ID: | 84971 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using ATG4D Rabbit pAb (CAB21690) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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