The ATG4D Polyclonal Antibody is a valuable tool for researchers studying ATG4D, a key protein involved in the autophagy process. Autophagy is a cellular mechanism essential for maintaining cellular homeostasis and is implicated in various diseases such as cancer, neurodegenerative disorders, and infections.This antibody, generated in rabbits, specifically targets the ATG4D protein and is highly reactive with human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of ATG4D in various cell types. This makes it an ideal choice for studies in the fields of cell biology, cancer research, and infectious diseases.
ATG4D plays a crucial role in the regulation of autophagy, a process that helps cells remove damaged organelles and proteins to maintain cellular health. Dysregulation of autophagy has been linked to the development of various diseases, making ATG4D a promising target for therapeutic interventions. Understanding the function of ATG4D can provide valuable insights into the mechanisms underlying these diseases and help in the development of novel treatment strategies.
Product Name:
ATG4D Polyclonal Antibody
SKU:
CAB21690
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84971
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATG4D Rabbit pAb (CAB21690) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
