The ATG4D Polyclonal Antibody (CAB7842) is a vital tool for researchers studying ATG4D, a key protein involved in autophagy regulation. Autophagy is a cellular process that degrades and recycles damaged organelles and proteins, playing a critical role in maintaining cellular health and function. The antibody, produced in rabbits, demonstrates high specificity and sensitivity when detecting ATG4D in human samples, making it a reliable tool for Western blot applications.ATG4D, a member of the autophagy-related 4 (ATG4) family, is essential for the processing of LC3 proteins during autophagosome formation. Dysregulation of ATG4D has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.
By targeting ATG4D with this polyclonal antibody, researchers can explore its function in autophagy and investigate potential therapeutic strategies for diseases associated with autophagy dysfunction.Overall, the ATG4D Polyclonal Antibody (CAB7842) provides a valuable tool for investigating the role of ATG4D in autophagy and its implications for human health and disease. Its high specificity and sensitivity make it a preferred choice for researchers conducting studies in the fields of cell biology, molecular biology, and biomedical research.
Product Name:
ATG4D Rabbit Polyclonal Antibody
SKU:
CAB7842
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-474 of human ATG4D (NP_116274.3).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84971
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skeletal muscle using ATG4D Rabbit pAb(CAB7842) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
