The ATG4D Polyclonal Antibody (CAB18097) is a valuable tool for researchers studying autophagy, a cellular process essential for maintaining cell homeostasis. This antibody, raised in rabbits, is highly specific for ATG4D and has been validated for use in various applications, including Western blotting and immunofluorescence.ATG4D is a key player in the autophagy pathway, responsible for cleaving the LC3/GABARAP proteins to promote autophagosome formation. Dysregulation of ATG4D has been implicated in various diseases, including neurodegenerative disorders and cancer.
By targeting ATG4D with this antibody, researchers can gain valuable insights into the mechanisms underlying autophagy and potentially identify new therapeutic targets for these diseases.Whether studying basic autophagy mechanisms or exploring the role of autophagy in disease pathogenesis, the ATG4D Polyclonal Antibody is a reliable tool for detecting and analyzing ATG4D expression in a variety of experimental systems. Its specificity and sensitivity make it a valuable addition to any researcher's toolkit in the fields of cell biology and molecular biology.
Product Name:
[KO Validated] ATG4D Rabbit Polyclonal Antibody
SKU:
CAB18097
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84971
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit pAb (CAB18097) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
