The ATG4C Polyclonal Antibody (CAB7396) is a valuable tool for researchers studying ATG4C, a key player in the autophagy process. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications. By binding to the ATG4C protein, this antibody allows for the detection and analysis of ATG4C in various cell types, offering insights into its role in autophagy regulation.ATG4C is a critical factor in the autophagy pathway, involved in the cleavage of the LC3 protein and the maturation of autophagosomes.
Dysregulation of ATG4C has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting the importance of understanding its function. By studying ATG4C using this antibody, researchers can gain valuable insights into the mechanisms underlying autophagy and its implications for disease pathology.
Product Name:
ATG4C Rabbit Polyclonal Antibody
SKU:
CAB7396
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ATG4C (NP_116241.2).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.
Purification Method:
Affinity purification
Gene ID:
84938
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG4C antibody (CAB7396) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
