Anti-ATG4B Antibody (CAB14112)

Antibody Name:	Anti-ATG4B Antibody
Antibody SKU:	CAB14112
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human ATG4B (NP_037457.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human ATG4B (NP_037457.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDAA TLTY DTLR FAEF EDFP ETSE PVWI LGRK YSIF TEKD EILS DVAS RLWF TYRK NFPA IGGT GPTS DTGW GCML RCGQ MIFA QALV CRHL GRDW RWTQ RKRQ PDSY FSVL NAFI DRKD SYYS IHQI AQMG VGEG KSIG QWYG PNTV AQVL KKLA VFDT WSSL AVHI AMDN TVVM EEIR RLCR TSVP CAGA TAFP ADSD RHCN GFPA GAEV TNRP SPWR PLVL LIPL RLGL TDIN EAYV ETLK HCFM MPQS LGVI GGKP NSAH YFIG YVGE ELIY LDPH TTQP AVEP TDGC FIPD ESFH CQHP PCRM SIAE LDPS IAVG FFCK TEDD FNDW CQQV KKLS LLGG ALPM FELV ELQP SHLA CPDV LNLS LDSS DVER LERF FDSE DEDF EILS L
Gene ID:	23192
Uniprot:	Q9Y4P1
Cellular Location:	Cytoplasm
Calculated MW:	31kDa/37kDa/42kDa/44kDa/52kDa
Observed MW:	44kDa

Synonyms:

ATG4B, APG4B, AUTL1

Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.22.-; Autophagy; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytoplasm; cytosol Molecular Function:cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; protein binding Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; macroautophagy; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis
NCBI Summary:	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y4P1
NCBI GenInfo Identifier:	296434400
NCBI Gene ID:	23192
NCBI Accession:	Q9Y4P1.2
UniProt Secondary Accession:	Q9Y4P1,Q53NU4, Q6ZUV8, Q8WYM9, Q96K07, Q96K96, Q96SZ1 Q9Y2F2, B7WNK2,
UniProt Related Accession:	Q9Y4P1
Molecular Weight:	42,622 Da
NCBI Full Name:	Cysteine protease ATG4B
NCBI Synonym Full Names:	autophagy related 4B cysteine peptidase
NCBI Official Symbol:	ATG4B
NCBI Official Synonym Symbols:	APG4B; AUTL1
NCBI Protein Information:	cysteine protease ATG4B
UniProt Protein Name:	Cysteine protease ATG4B
UniProt Synonym Protein Names:	AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B
Protein Family:	Cysteine protease
UniProt Gene Name:	ATG4B
UniProt Entry Name:	ATG4B_HUMAN

Western blot analysis of extracts of rat brain, using ATG4B antibody (CAB14112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.

Antibodies

Anti-ATG4B Antibody (CAB2837)

Anti-Atg4B Antibody (CAB5059)

ATG4B Antibody (PACO02758)

ATG4B Antibody (PACO07307)

ATG4B Antibody (PACO07903)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)