The ATG4B Polyclonal Antibody is a valuable tool for researchers studying ATG4B, a key regulator of autophagy, the cellular process responsible for degrading and recycling damaged or unnecessary cellular components. This antibody is raised in rabbits and is highly specific for human samples, making it ideal for use in Western blot applications. By binding to the ATG4B protein, this antibody allows for the detection and analysis of ATG4B levels in various cell types, providing important insights into the autophagy pathway.ATG4B is a crucial player in the autophagy process, as it is responsible for cleaving and processing the LC3 protein, which is essential for forming autophagosomes.
Dysregulation of ATG4B has been implicated in a variety of diseases, including cancer, neurodegenerative disorders, and infectious diseases. By studying ATG4B using this antibody, researchers can gain a better understanding of the autophagy pathway and potentially identify new therapeutic targets for treating these diseases.Overall, the ATG4B Polyclonal Antibody is a valuable tool for researchers interested in autophagy and its role in various disease processes. Its specificity for human samples and reliability in Western blot applications make it a versatile option for studies in cell biology, cancer research, and beyond.
Product Name:
ATG4B Polyclonal Antibody
SKU:
CAB21409
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human ATG4B (NP_037457.3).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
23192
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG4B antibody (CAB21409) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
