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ATG4A Polyclonal Antibody (CAB21356)

SKU:
CAB21356
$155
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Description

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Product Name:ATG4A Polyclonal Antibody
SKU:CAB21356
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).
Sequence:TEEN GTVN DQTF HCLQ SPQR MNIL NLDP SVAL GFFC KEEK DFDN WCSL VQKE ILKE NLRM FELV QKHP SHWP PFVP PAKP EVTT TGAE FIDS TEQL EEFD LEED FEIL SV
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:APG4A; AUTL2; HsAPG4A; ATG4A
Positive Sample:Jurkat,K-562,HeLa,Jurkat,HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:45kDa
Observed MW:45kDa/50kDa

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Purification Method:Affinity purification
Gene ID:115201
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] ATG4A Rabbit pAb (CAB21356) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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