The ATG4A Polyclonal Antibody is a valuable tool for research involving ATG4A, a key player in the autophagy pathway. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it ideal for studies in autophagy, cell biology, and disease mechanisms. ATG4A is a cysteine protease that regulates autophagosome formation by cleaving the LC3/GABARAP proteins. Dysregulation of ATG4A has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. Understanding the role of ATG4A in autophagy is crucial for developing targeted therapies that aim to modulate this pathway in disease contexts.
This ATG4A Polyclonal Antibody is validated for use in Western blot applications, enabling precise detection and analysis of ATG4A protein levels in different cell types and tissues. By utilizing this antibody, researchers can deepen their understanding of the autophagy process and its implications in disease pathology, ultimately leading to the development of novel treatment strategies.
Product Name:
ATG4A Polyclonal Antibody
SKU:
CAB21356
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Purification Method:
Affinity purification
Gene ID:
115201
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] ATG4A Rabbit pAb (CAB21356) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
