The ATG3 Polyclonal Antibody (CAB5809) is a powerful tool for researchers studying autophagy, a vital cellular process involved in the degradation and recycling of cellular components. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.ATG3 is a key player in the autophagy pathway, responsible for facilitating the conjugation of ATG8 to phosphatidylethanolamine on the autophagosome membrane. By targeting ATG3 with this polyclonal antibody, researchers can visualize and quantify ATG3 protein levels in various cell types, aiding in the investigation of autophagy-related processes.
Understanding the role of ATG3 in autophagy is crucial for elucidating its involvement in various diseases, including cancer, neurodegenerative disorders, and metabolic conditions. By harnessing the power of the ATG3 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying autophagy and its impact on cellular homeostasis.
Product Name:
ATG3 Rabbit Polyclonal Antibody
SKU:
CAB5809
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2).
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
64422
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG3 antibody (CAB5809) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
