The ATG14 Polyclonal Antibody (CAB7526) is a valuable tool for researchers studying ATG14, a critical component of the autophagy pathway involved in maintaining cellular homeostasis and responding to stress. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ATG14 protein, it enables accurate detection and analysis in a variety of cell types, making it a key asset for investigations in the fields of cell biology, metabolism, and cancer research.ATG14, also known as Beclin 1-associated autophagy-related key regulator, plays a key role in the formation of autophagosomes and the initiation of the autophagy process.
Dysregulation of autophagy has been linked to a range of diseases, including cancer, neurodegenerative disorders, and metabolic conditions. By studying the function and regulation of ATG14, researchers can gain important insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention. The ATG14 Polyclonal Antibody is a valuable tool for advancing our understanding of autophagy and its implications for human health.
Product Name:
ATG14 Rabbit Polyclonal Antibody
SKU:
CAB7526
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 340-440 of human ATG14 (NP_055739.2).
Enables GTPase binding activity. Involved in several processes, including macroautophagy; regulation of phosphorylation; and response to mitochondrial depolarisation. Acts upstream of or within endosome to lysosome transport. Located in autophagosome and phagophore assembly site membrane. Is extrinsic component of omegasome membrane and extrinsic component of phagophore assembly site membrane.
Purification Method:
Affinity purification
Gene ID:
22863
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG14 antibody (CAB7526) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
