The ATG12 Rabbit Monoclonal Antibody (CAB19610) is a valuable tool for researchers studying the ATG12 protein, a key player in the autophagy pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blotting and immunofluorescence applications.Autophagy is a crucial cellular process that helps maintain cellular homeostasis by degrading damaged organelles and proteins. Dysregulation of autophagy has been linked to various diseases, including cancer and neurodegenerative disorders. ATG12 is an essential component of the autophagy machinery, playing a critical role in the elongation of the autophagosome membrane.
By utilizing the ATG12 Rabbit Monoclonal Antibody, researchers can investigate the expression and localization of ATG12 in different cell types and experimental conditions. This antibody enables the detection and analysis of ATG12, providing valuable insights into the autophagy process and its implications for disease pathology. Studying ATG12 with this antibody may lead to a better understanding of autophagy-related diseases and the development of novel therapeutic strategies targeting the autophagy pathway.
Product Name:
ATG12 Rabbit Monoclonal Antibody
SKU:
CAB19610
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 41-140 of human Apg12 (O94817).
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Purification Method:
Affinity purification
Gene ID:
9140
Clone Number:
ARC0089
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG12 antibody (CAB19610) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
