The ATG12 Polyclonal Antibody (CAB15609) is a valuable tool for research involving the ATG12 protein, an important player in the process of autophagy. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ATG12 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studying autophagy and its role in various physiological and pathological processes.ATG12 is a key component of the autophagy pathway, a cellular process crucial for maintaining cellular homeostasis by degrading damaged organelles and proteins.
Dysregulation of autophagy has been implicated in a range of diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Understanding the function of ATG12 is therefore essential for unraveling the mechanisms underlying these conditions and developing targeted therapies to intervene in autophagy-related pathways.By using the ATG12 Polyclonal Antibody in your research, you can gain valuable insights into the role of autophagy in health and disease, paving the way for innovative approaches to treating a variety of human disorders.
Product Name:
ATG12 Rabbit Polyclonal Antibody
SKU:
CAB15609
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG12 (NP_004698.3).
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).
Purification Method:
Affinity purification
Gene ID:
9140
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG12 antibody (CAB15609) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
