The ATG10 Rabbit Monoclonal Antibody (CAB6848) is a highly specific antibody designed for research involving the ATG10 protein, a key player in the autophagy pathway. This antibody, raised in rabbits, is validated for use in various applications such as Western blot, immunohistochemistry, and immunofluorescence, allowing for the detection and analysis of ATG10 in different cell types and tissues.ATG10 is a critical component of the autophagy process, responsible for the conjugation of ATG12 to ATG5, which is essential for the formation of the autophagosome. Dysregulation of autophagy has been linked to various diseases including cancer, neurodegenerative disorders, and metabolic conditions.
Therefore, studying ATG10 and its role in autophagy can provide valuable insights into the underlying mechanisms of these diseases and potentially lead to the development of novel therapeutic strategies.Overall, the ATG10 Rabbit Monoclonal Antibody (CAB6848) is a reliable tool for researchers interested in exploring the role of ATG10 in autophagy and its significance in various disease contexts. Its high specificity and sensitivity make it an ideal choice for studies aiming to further our understanding of the autophagy pathway and its implications in health and disease.
Product Name:
ATG10 Rabbit Monoclonal Antibody
SKU:
CAB6848
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 121-220 of human ATG10 (Q9H0Y0).
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Purification Method:
Affinity purification
Gene ID:
83734
Clone Number:
ARC1425
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (CAB6848) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
