Anti-ATG10 Antibody (CAB7390)

Antibody Name:	Anti-ATG10 Antibody
Antibody SKU:	CAB7390
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ATG10 (NP_113670.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ATG10 (NP_113670.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEED EFIG EKTF QRYC AEFI KHSQ QIGD SWEW RPSK DCSD GYMC KIHF QIKN GSVM SHLG ASTH GQTC LPME EAFE LPLD DCEV IETA AASE VIKY EYHV LYSC SYQV PVLY FRAS FLDG RPLT LKDI WEGV HECY KMRL LQGP WDTI TQQE HPIL GQPF FVLH PCKT NEFM TPVL KNSQ KINK NVNY ITSW LSIV GPVV GLNL PLSY AKAT SQDE RNVP
Gene ID:	83734
Uniprot:	Q9H0Y0
Cellular Location:	Cytoplasm
Calculated MW:	14kDa/25kDa
Observed MW:	25kDa

Synonyms:	ATG10, APG10, APG10L, pp12616
Background:

UniProt Protein Function:	ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Autophagy; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: cytosol; intracellular Molecular Function:APG12 conjugating enzyme activity Biological Process: autophagy; macroautophagy; positive regulation of protein modification process; protein amino acid lipidation; protein modification by small protein conjugation
NCBI Summary:	Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9H0Y0
NCBI GenInfo Identifier:	62286633
NCBI Gene ID:	83734
NCBI Accession:	Q9H0Y0.1
UniProt Secondary Accession:	Q9H0Y0,Q6PIX1, Q9H842, B2RE09,
UniProt Related Accession:	Q9H0Y0
Molecular Weight:	14,217 Da
NCBI Full Name:	Ubiquitin-like-conjugating enzyme ATG10
NCBI Synonym Full Names:	autophagy related 10
NCBI Official Symbol:	ATG10
NCBI Official Synonym Symbols:	APG10; APG10L; pp12616
NCBI Protein Information:	ubiquitin-like-conjugating enzyme ATG10
UniProt Protein Name:	Ubiquitin-like-conjugating enzyme ATG10
UniProt Synonym Protein Names:	Autophagy-related protein 10; APG10-like
Protein Family:	Ubiquitin-like-conjugating enzyme
UniProt Gene Name:	ATG10
UniProt Entry Name:	ATG10_HUMAN

	Western blot analysis of extracts of mouse small intestine, using ATG10 antibody (CAB7390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human colon using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse spleen using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat testis using ATG10 Rabbit pAb (CAB7390) at dilution of 1:100 (40x lens).

Antibodies

Anti-ATG10 Antibody (CAB6848)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)