The ATG10 Polyclonal Antibody (CAB7390) is a powerful tool for researchers studying autophagy, a critical cellular process involved in maintaining cellular homeostasis and protecting against various diseases. This antibody, produced in rabbits, specifically targets the ATG10 protein, a key player in the formation of the autophagosome.With its high reactivity with human samples, the ATG10 Polyclonal Antibody is ideal for use in Western blot applications, allowing for the detection and analysis of ATG10 in a variety of cell types.
By studying the role of ATG10 in autophagy, researchers can gain insights into its involvement in diseases such as cancer, neurodegenerative disorders, and metabolic disorders.Given the importance of autophagy in health and disease, understanding the function of ATG10 is essential for developing targeted therapies that manipulate autophagic pathways for therapeutic benefit. The ATG10 Polyclonal Antibody provides researchers with a valuable tool to advance our understanding of autophagy and its impact on human health.
Product Name:
ATG10 Rabbit Polyclonal Antibody
SKU:
CAB7390
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 120-220 of human ATG10 (NP_113670.1).
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Purification Method:
Affinity purification
Gene ID:
83734
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse small intestine, using ATG10 Rabbit pAb (CAB7390) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
