The ATF7 Polyclonal Antibody (CAB7580) is a valuable tool for researchers studying ATF7, a transcription factor involved in gene regulation and cellular processes. This antibody, produced in rabbits, has high specificity for ATF7 and is suitable for use in Western blot applications. By binding to ATF7 protein, this antibody enables the detection and analysis of ATF7 expression in various cell types, making it ideal for investigations in molecular biology and cancer research.
ATF7, also known as activating transcription factor 7, plays a key role in regulating gene expression and cell proliferation. Its involvement in various signaling pathways and cellular functions makes it a promising target for studying diseases such as cancer, diabetes, and neurodegenerative disorders. Understanding the role of ATF7 can provide insights into disease mechanisms and potential therapeutic strategies targeting this transcription factor.
Product Name:
ATF7 Rabbit Polyclonal Antibody
SKU:
CAB7580
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human ATF7 (NP_001193611.1).
Sequence:
MGDD RPFV CNAP GCGQ RFTN EDHL AVHK HKHE MTLK FGPA RTDS VIIA DQTP TPTR FLKN CEEV GLFN ELAS SFEH EFKK AADE DEKK ARSR TVAK KLVV FRPR LFLL CFGI IFLI G
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
ATFA; ATF7
Positive Sample:
BT-474,SW620,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, nucleoplasm.
Calculated MW:
52kDa
Observed MW:
53kDa
Enables several functions, including DNA-binding transcription repressor activity, RNA polymerase II-specific; mitogen-activated protein kinase binding activity; and transcription coactivator binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of colorectal cancer.
Purification Method:
Affinity purification
Gene ID:
11016
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ATF7 antibody (CAB7580) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
