The ATF6 Polyclonal Antibody (CAB12570) is a valuable tool for research involving ATF6, a key transcription factor involved in the unfolded protein response (UPR) pathway. The antibody, produced in rabbits, is highly specific for human ATF6 and has been validated for use in Western blot and immunofluorescence applications. By binding to ATF6, this antibody enables researchers to detect and analyze the protein in various cell types, making it an essential component in studies related to endoplasmic reticulum stress, protein folding, and cellular homeostasis.ATF6 is a crucial regulator of UPR, a cellular mechanism that responds to the accumulation of unfolded proteins in the endoplasmic reticulum.
Dysregulation of this pathway has been implicated in various diseases, including neurodegenerative disorders, diabetes, and cardiovascular diseases. By understanding the role of ATF6 in UPR, researchers can gain insights into disease mechanisms and identify potential therapeutic targets for intervention. The ATF6 Polyclonal Antibody provides a reliable tool for studying ATF6 function and its implications in health and disease.
Product Name:
ATF6 Polyclonal Antibody
SKU:
CAB12570
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600-670 of human ATF6 (NP_031374.2).
Sequence:
INEN VING QDYE VMMQ IDCQ VMDT RILH IKSS SVPP YLRD QQRN QTNT FFGS PPAA TEAT HVVS TIPE SLQ
Endoplasmic reticulum membrane, Nucleus, Single-pass type II membrane protein.
Calculated MW:
75kDa
Observed MW:
92kDa
This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis.
Purification Method:
Affinity purification
Gene ID:
22926
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATF6 Rabbit pAb (CAB12570) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
