The ATF4 Polyclonal Antibody is a valuable tool for researchers studying ATF4, a key transcription factor involved in cellular stress responses and the unfolded protein response. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to ATF4 protein, this antibody allows for the detection and analysis of ATF4 expression in a variety of cell types, making it an essential tool for studies in molecular biology, cell signaling, and cancer research.ATF4 is a crucial regulator of cellular processes such as metabolism, apoptosis, and the response to oxidative stress. Its role in coordinating the adaptive response to environmental challenges has made it a target of interest in the fields of cancer biology, neurodegenerative diseases, and metabolic disorders.
Research into the function and regulation of ATF4 is essential for understanding how cells adapt to stress and for developing therapeutic strategies to target ATF4-dependent pathways.Overall, the ATF4 Polyclonal Antibody is an essential reagent for researchers investigating the role of ATF4 in cellular stress responses and disease pathogenesis. Its high specificity and sensitivity make it a reliable tool for studying ATF4 expression and activity in various experimental settings, paving the way for a better understanding of ATF4 function and its implications in human health and disease.
Product Name:
ATF4 Polyclonal Antibody
SKU:
CAB21500
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-351 of human ATF4 (NP_877962.1).
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
Purification Method:
Affinity purification
Gene ID:
468
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using ATF4 Rabbit pAb (CAB21500) at 1:500 dilution.C2C12 cells were treated by tunicamycin (2 μg/ml) for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
