The ATF4 Polyclonal Antibody (CAB0201) is a valuable tool for researchers studying ATF4, a transcription factor involved in the regulation of various cellular processes, including stress response and amino acid metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to ATF4 protein, this antibody allows for accurate detection and analysis in a range of cell types, making it well-suited for investigations in molecular biology and cancer research.ATF4 is known for its role in coordinating cellular responses to stress, such as nutrient deprivation and oxidative stress, by controlling the expression of genes involved in pathways like autophagy and amino acid metabolism.
Dysregulation of ATF4 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic disorders. By studying ATF4 activity, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the ATF4 Polyclonal Antibody (CAB0201) serves as a valuable tool for researchers seeking to explore the function of ATF4 in cellular processes and disease states, ultimately contributing to advancements in the understanding and treatment of various health conditions.
Product Name:
ATF4 Rabbit Polyclonal Antibody
SKU:
CAB0201
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
Purification Method:
Affinity purification
Gene ID:
468
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using ATF4 Rabbit pAb (CAB0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
