The ATF3 Polyclonal Antibody (CAB1852) is a valuable tool for researchers studying ATF3, a transcription factor involved in stress responses and regulation of gene expression. This antibody, produced in rabbits, shows high reactivity with ATF3 in human samples and is suitable for use in Western blot applications. By specifically binding to ATF3, this antibody enables researchers to detect and analyze the protein in various cell types, making it an important asset for studies in molecular biology and stress-related pathways.ATF3, also known as activating transcription factor 3, is a key player in cellular stress responses and is involved in the regulation of genes that control cell growth, differentiation, and apoptosis.
Its role in modulating gene expression in response to various stress stimuli makes it a promising target for research into cancer, neurodegenerative diseases, and inflammatory conditions. Investigating the function of ATF3 can provide insights into the mechanisms underlying these diseases and potentially lead to the development of novel therapeutic strategies.
Product Name:
ATF3 Rabbit Polyclonal Antibody
SKU:
CAB1852
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
Purification Method:
Affinity purification
Gene ID:
467
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW 264.7 cells using ATF3 Rabbit pAb (CAB1852) at 1:1000 dilution incubated overnight at 4°C. Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 180 s.
