The ATF1 Polyclonal Antibody (CAB5791) is a valuable tool for researchers studying ATF1, a transcription factor involved in regulating gene expression and cell proliferation. This antibody is produced in rabbits and is highly specific for ATF1 in human samples, making it ideal for Western blot applications. By binding to ATF1, this antibody enables researchers to detect and analyze ATF1 protein levels in various cell types, facilitating studies in cancer biology and gene regulation.ATF1 plays a crucial role in controlling the expression of genes involved in cell growth, differentiation, and survival, making it a key player in cellular processes like proliferation and apoptosis.
Dysregulation of ATF1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. Understanding the function of ATF1 can provide valuable insights into disease mechanisms and potential therapeutic targets for intervention.Overall, the ATF1 Polyclonal Antibody (CAB5791) is a reliable tool for researchers seeking to investigate the role of ATF1 in cellular processes and disease pathogenesis. Its specificity and sensitivity make it a valuable asset in the field of molecular biology and cancer research.
Product Name:
ATF1 Rabbit Polyclonal Antibody
SKU:
CAB5791
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human ATF1 (NP_005162.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TREB36; EWS-ATF1; FUS/ATF-1; ATF1
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
29kDa
Observed MW:
36kDa
This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
466
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using ATF1 Rabbit pAb (CAB5791) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
