The Asymmetric Dimethyl Histone H4 R3 Polyclonal Antibody (CAB7261) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, raised in rabbits, specifically targets and binds to the histone H4 protein that is asymmetrically dimethylated at the arginine 3 residue.Histone modifications, such as asymmetric dimethylation, play a crucial role in regulating gene expression and chromatin structure. By targeting this specific modification on histone H4, researchers can gain insights into how epigenetic changes impact gene regulation and cellular processes.Validated for use in various applications, including Western blotting and immunofluorescence, this antibody allows for the detection and analysis of asymmetric dimethyl histone H4 R3 in different biological samples.
Its high reactivity with human samples makes it a valuable tool for studies in epigenetics, cancer research, and other fields where chromatin modifications are of interest.Understanding the role of asymmetric dimethyl histone H4 R3 in gene regulation and disease processes is essential for developing targeted therapies and advancing our knowledge of epigenetic mechanisms. This antibody provides researchers with a reliable tool to investigate these important biological processes.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Asymmetric DiMethyl-Histone H4-R3 Rabbit pAb (CAB7261) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 300s.
