The Asymmetric Dimethyl Histone H4 (R3) Polyclonal Antibody (CAB2376) is a specially designed antibody for research involving histone modifications, specifically targeting the asymmetric dimethylation of histone H4 at arginine 3 (H4R3me2a). This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in multiple applications, including Western blot and immunofluorescence.Histone modifications, such as asymmetric dimethylation of histone H4, play a crucial role in regulating gene expression and chromatin structure. The detection and analysis of these modifications are essential in understanding processes like transcriptional regulation, DNA repair, and cell differentiation.
The CAB2376 antibody specifically binds to H4R3me2a, allowing for accurate detection and visualization of this important histone modification in various cell types.Researchers in the fields of epigenetics, chromatin biology, and cancer research will find this antibody invaluable for studying the role of histone modifications in disease development and progression. The ability to analyze asymmetric dimethyl histone H4 (R3) with high specificity and sensitivity makes the CAB2376 antibody a valuable tool for unraveling the complex mechanisms of epigenetic regulation and its impact on cellular function.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H4-R3 Rabbit pAb (CAB2376) at dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
