The Asymmetric Dimethyl Histone H3 (R8) Polyclonal Antibody (CAB3157) is a valuable tool for researchers studying epigenetic modifications and histone biology. This antibody is raised in rabbits and is highly specific for detecting the asymmetric dimethylation of histone H3 at arginine 8. It has been validated for use in various applications, including Western blot and immunohistochemistry, and is particularly reactive with human samples.Histone modifications, such as asymmetric dimethylation, play a crucial role in regulating gene expression and chromatin structure. The detection of this specific modification allows researchers to investigate its involvement in various cellular processes, including transcriptional regulation, DNA repair, and cell differentiation.
Understanding the impact of asymmetric dimethylation of histone H3 at arginine 8 can provide insights into the mechanisms underlying diseases like cancer, neurological disorders, and developmental abnormalities.By using the Asymmetric Dimethyl Histone H3 (R8) Polyclonal Antibody, researchers can analyze the distribution and levels of this histone modification in different cell types and tissues. This antibody enables the detection and quantification of asymmetric dimethyl histone H3 (R8), allowing for in-depth studies on epigenetic regulation and its implications in various biological processes.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB3157) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
