The Asymmetric Dimethyl Histone H3 R2 Polyclonal Antibody (CAB3155) is a valuable tool for researchers studying epigenetics and chromatin modifications. This antibody, generated in rabbits, specifically targets the histone H3 R2 modification, which plays a critical role in gene expression regulation and chromatin dynamics.Histone modifications, such as asymmetric dimethylation of H3 R2, have been linked to various cellular processes, including transcriptional activation and repression. The CAB3155 antibody is highly reactive with human samples and has been validated for use in Western blot applications, enabling researchers to study the dynamics of H3 R2 modification in different cell types and biological contexts.
By targeting the asymmetric dimethyl histone H3 R2 modification, this antibody allows for the detection and analysis of epigenetic changes that may be relevant in disease processes such as cancer development, cellular differentiation, and genomic stability. Researchers in the fields of epigenetics, cancer biology, and developmental biology will find this antibody essential for their studies on chromatin regulation and gene expression control.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (CAB3155) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
