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AST Fluorometric Activity Assay Kit

SKU:
MAES0253
Product Type:
Assay
Instrument:
Fluorescence Microplate Reader
Detection Method:
Fluorometric
Sample Type:
Cell, Animal Tissue, Serum, Plasma And Other Liquid Samples
Sensitivity:
0.03 U/L
Range:
0.03-1.5 U/L
Assay Time:
80 min
€469
Frequently bought together:

Description

Product Name:AST Fluorometric Activity Assay Kit
Product Code:MAES0253
Sample Type:Cell, Animal Tissue, Serum (Plasma) And Other Liquid Samples
Assay Time:80 min
Assay Type:Enzyme Activity
Synonyms:AST、GOT
Detection Instrument:Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Detection Method:Fluorescence method
Sensitivity:0.03 U/L
Detection Range:0.03-1.5 U/L
Inter-assay CV:0.073
Intra-assay CV:0.022

AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Micropipette, Vortex mixer, Centrifuge

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