The ASPh Polyclonal Antibody (CAB13153) is a valuable tool for researchers studying ASPh, a key enzyme involved in protein synthesis regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ASPh protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for research in the fields of molecular biology and biochemistry.ASPh, also known as aspartate-beta-hydroxylase, plays a crucial role in the post-translational modification of proteins, particularly in the regulation of protein stability and function.
Its involvement in essential cellular processes makes ASPh an intriguing target for investigations into various diseases, including cancer and neurological disorders. Understanding the mechanisms by which ASPh functions can provide valuable insights for developing targeted therapies and treatments in these areas of research.
Product Name:
ASPH Rabbit Polyclonal Antibody
SKU:
CAB13153
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 111-270 of human ASPH (NP_001158227.1).
Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane, Single-pass type II membrane protein.
Calculated MW:
86kDa
Observed MW:
86kDa
This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.
Purification Method:
Affinity purification
Gene ID:
444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ASPH Rabbit pAb (CAB13153) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
