The ASCL4 Polyclonal Antibody (CAB14439) is a vital tool for researchers studying the ASCL4 protein, a key transcription factor involved in neurogenesis and neuronal differentiation. This antibody, developed in rabbits, exhibits high specificity for human samples and has been validated for use in various applications, including Western blotting.ASCL4, also known as Achaete-scute homolog 4, plays a crucial role in the development of the nervous system by regulating the differentiation of neural progenitor cells. Its involvement in neurogenesis makes it a promising target for research in neurobiology and developmental biology.
Understanding the function of ASCL4 can provide valuable insights into neuronal development and the underlying mechanisms of neurological disorders.By utilizing the ASCL4 Polyclonal Antibody, researchers can accurately detect and analyze ASCL4 expression in different cell types, enhancing their ability to study neurogenesis and neuronal differentiation. This antibody is essential for investigations in neuroscience, stem cell biology, and regenerative medicine, where ASCL4's role in neural development is of significant interest.
Product Name:
ASCL4 Rabbit Polyclonal Antibody
SKU:
CAB14439
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human ASCL4 (NP_982260.3).
Sequence:
METR KPAE RLAL PYSL RTAP LGVP GTLP GLPR RDPL RVAL RLDA ACWE WARS GCAR GWQY LPVP LDSA FE
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:100
Synonyms:
ASH-4; HASH4; bHLHa44; ASCL4
Positive Sample:
Mouse brain,Mouse kidney,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
19kDa
Observed MW:
19kDa
Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).
Purification Method:
Affinity purification
Gene ID:
121549
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ASCL4 Rabbit pAb (CAB14439) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
