The ASCL2 Polyclonal Antibody (PAC021935) is a valuable tool for researchers studying ASCL2, a transcription factor that plays a crucial role in stem cell maintenance and differentiation. This antibody is raised in rabbits and has been validated for use in various applications, including Western blotting and immunohistochemistry.ASCL2, also known as achaete-scute family bHLH transcription factor 2, is involved in the regulation of gene expression in stem cells, particularly in the intestine and colon. Its dysregulation has been linked to various diseases, including colorectal cancer and gastrointestinal disorders.
By using the PAC021935 antibody, researchers can accurately detect and analyze ASCL2 expression in different cell types, shedding light on its function and potential therapeutic targets.Furthermore, understanding the role of ASCL2 in stem cell biology and disease pathology could lead to the development of novel treatments for a variety of conditions, making the PAC021935 antibody an invaluable tool for researchers in the fields of molecular biology and cancer research.
Antibody Name:
ASCL2 Antibody (PACO21935)
Antibody SKU:
PACO21935
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from Internal of human ASCL2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from K562 cells, using ASCL2 antibody.
Background:
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. Westerman B.A., Placenta 22:511-518(2001).
ASCL2: a basic helix-loop-helix transcription factor, plays an essential role in the maintenance of adult intestinal stem cellsProtein type: DNA-bindingChromosomal Location of Human Ortholog: 11p15.5Cellular Component: cytoplasm; nucleusBiological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of neurogenesis; response to hypoxia; transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. [provided by RefSeq, Jul 2008]
