The ASAP2 Polyclonal Antibody (CAB17579) is a valuable tool for researchers studying the ASAP2 protein, a crucial player in cell signaling pathways and cancer progression. Raised in rabbits, this antibody is highly specific and reactive to human samples, making it ideal for use in Western blot applications. By binding to the ASAP2 protein, researchers can easily detect and analyze its expression in various cell types, providing important insights into its role in cancer biology.ASAP2, also known as Arf-GAP with SH3 domain, ankyrin repeat and PH domain-containing protein 2, is involved in regulating cell migration, adhesion, and proliferation, making it a promising target for cancer therapy.
Its dysregulation has been linked to tumor growth and metastasis, highlighting its importance in cancer research. By studying ASAP2 with the help of this antibody, researchers can further understand its involvement in cancer development and potentially identify new therapeutic strategies to combat the disease.
Product Name:
ASAP2 Rabbit Polyclonal Antibody
SKU:
CAB17579
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human ASAP2 (NP_001128663).
This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8853
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ASAP2 Rabbit pAb (CAB17579) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
