The ASAH2 Polyclonal Antibody (CAB12596) is a valuable tool for research involving ASAH2, a key enzyme involved in ceramide metabolism. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications such as Western blotting. By binding specifically to the ASAH2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in lipid metabolism and related fields.ASAH2, also known as acid ceramidase, plays a crucial role in regulating cellular levels of ceramide, a bioactive lipid involved in processes such as apoptosis, inflammation, and cellular signaling.
Dysregulation of ASAH2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Investigating the function of ASAH2 is essential for gaining insights into these conditions and developing potential therapeutic strategies targeting ceramide metabolism.
Product Name:
ASAH2 Rabbit Polyclonal Antibody
SKU:
CAB12596
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1).
Cell membrane, Single-pass type II membrane protein.
Calculated MW:
86kDa
Observed MW:
85kDa
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
Purification Method:
Affinity purification
Gene ID:
56624
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ASAH2 antibody (CAB12596) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
