The ASAH1 Polyclonal Antibody (CAB13948) is a valuable tool for researchers studying ASAH1, an enzyme involved in the metabolism of sphingolipids and cellular signaling pathways. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the ASAH1 protein, researchers can accurately detect and analyze the expression of ASAH1 in a variety of cell types, making it ideal for studies in lipid metabolism, signal transduction, and cancer research.
ASAH1, also known as acid ceramidase, plays a crucial role in maintaining cellular homeostasis by regulating the levels of ceramides and sphingosine within cells. Dysregulation of ASAH1 activity has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. Studying the function of ASAH1 is essential for gaining insights into these diseases and developing targeted therapies aimed at modulating lipid metabolism and cellular signaling pathways.
Product Name:
ASAH1 Rabbit Polyclonal Antibody
SKU:
CAB13948
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 140-389 of human ASAH1 (NP_001120977.1).
This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy.
Purification Method:
Affinity purification
Gene ID:
427
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from RT4 cells using ASAH1 Rabbit pAb(CAB13948) at 1:700 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:60s.
