The ARSI Antibody (PAC023387) is a valuable tool for researchers studying the arylsulfatase I (ARSI) protein, which is involved in the metabolism of sulfated steroids and other molecules. This antibody, produced by Assay Genie, is a polyclonal antibody raised in rabbits, ensuring high reactivity and specificity for human samples. It has been validated for use in various applications, including Western blot, immunohistochemistry, and ELISA.ARSI is a crucial enzyme involved in the hydrolysis of sulfate esters, playing a role in the regulation of steroid hormones and other bioactive molecules. Dysregulation of ARSI activity has been implicated in various diseases, including prostate cancer and other hormone-related disorders.
By targeting ARSI with this specific antibody, researchers can study its expression and activity in different cell types and tissues, providing valuable insights into its function and potential therapeutic applications.Overall, the ARSI Antibody is a versatile tool for scientists conducting research in molecular biology, biochemistry, and endocrinology. Its reliability and specificity make it a valuable asset for understanding the role of ARSI in health and disease, paving the way for new developments in targeted therapies and diagnostic approaches.
Synthesized peptide derived from internal of human ARSI.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COS7 cells, using ARSI antibody.
Immunofluorescence analysis of MCF-7 cells, using ARSI antibody.
Background:
Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts.
Synonyms:
arylsulfatase I; ASI; EC 3.1.6.-;
UniProt Protein Function:
ARSI: Displays arylsulfatase activity at neutral pH, when co- expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted, signal peptide; Hydrolase; Secreted; Calcium-binding; EC 3.1.6.-Chromosomal Location of Human Ortholog: 5q32Cellular Component: endoplasmic reticulum lumen; extracellular regionMolecular Function: arylsulfatase activity; metal ion binding; protein bindingBiological Process: cellular protein metabolic process; glycosphingolipid metabolic process; post-translational protein modification; sphingolipid metabolic process
UniProt Protein Details:
NCBI Summary:
Sulfatases (EC 3.1.5.6), such as ARSI, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM, Mar 2008]
