The ARNT Polyclonal Antibody (CAB14705) is a valuable tool for researchers studying the AhR-ARNT signaling pathway, involved in various cellular processes including xenobiotic metabolism and cancer development. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the ARNT protein, this antibody enables precise detection and analysis in a range of cell types, making it a versatile tool for studies in molecular biology, toxicology, and oncology.ARNT, also known as HIF-1β, is a key transcription factor that forms heterodimers with other proteins, including AhR and HIF-1α, to regulate gene expression in response to environmental stimuli such as hypoxia and chemical exposure.
Dysregulation of the AhR-ARNT pathway has been implicated in various diseases, including cancer, metabolic disorders, and cardiovascular disease. Understanding the role of ARNT in these processes is essential for developing targeted therapies and interventions that modulate gene expression for therapeutic benefit.
Product Name:
HIF1β/ARNT Rabbit Polyclonal Antibody
SKU:
CAB14705
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HIF1β/ARNT (NP_001659.1).
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
405
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat uterus, using HIF1β/ARNT antibody (CAB14705) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
