The ARL8B Polyclonal Antibody (CAB8869) is a valuable tool for researchers studying the protein ARL8B, which plays a key role in regulating membrane trafficking and lysosome positioning within cells. This antibody, raised in rabbits, is highly specific and reactive with human, mouse, and rat samples, making it versatile for a variety of experimental applications.ARL8B is a member of the ADP-ribosylation factor-like family and is known to be involved in processes such as endocytic trafficking, phagosome maturation, and autophagy. By targeting ARL8B, researchers can study its function in these cellular processes and gain insights into its potential role in diseases like cancer, neurodegenerative disorders, and infectious diseases.
The ARL8B Polyclonal Antibody is validated for use in Western blot, immunofluorescence, and immunohistochemistry applications, providing researchers with multiple options for detecting and visualizing ARL8B in various cell types and tissues. Its high specificity and sensitivity make it an essential tool for advancing research in cell biology and understanding the molecular mechanisms underlying cellular trafficking pathways.
Product Name:
ARL8B Rabbit Polyclonal Antibody
SKU:
CAB8869
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-186 of human ARL8B (NP_060654.1).
Cytoplasm, Late endosome membrane, Lysosome membrane, cytoskeleton, spindle.
Calculated MW:
22kDa
Observed MW:
21kDa
Enables guanyl ribonucleotide binding activity and tubulin binding activity. Involved in several processes, including calcium ion regulated lysosome exocytosis; lysosomal transport; and vesicle fusion. Located in cytolytic granule membrane; midbody; and spindle midzone. Colocalizes with lysosomal membrane.
Purification Method:
Affinity purification
Gene ID:
55207
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ARL8B Rabbit pAb (CAB8869) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
