The ARID1A Polyclonal Antibody (CAB18650) is a valuable tool for researchers studying ARID1A, a key player in chromatin remodeling and gene expression regulation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the ARID1A protein, allowing for precise detection and analysis in various cell types, making it ideal for studies in cancer, developmental biology, and epigenetics.
ARID1A, a member of the SWI/SNF chromatin remodeling complex, is known to be a tumor suppressor gene in various cancers. Its role in regulating gene expression and chromatin architecture makes it a critical target for research into cancer development and progression. Understanding the function of ARID1A can provide valuable insights into the mechanisms underlying cancer and potential therapeutic targets for treatment.
Product Name:
ARID1A Rabbit Polyclonal Antibody
SKU:
CAB18650
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2195-2285 of human ARID1A (NP_006006.3).
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8289
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of C2C12, using ARID1A Rabbit pAb (CAB18650) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
