ARHGEF18 Antibody (PACO31944)
- SKU:
- PACO31944
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
ARHGEF18 Antibody (PACO31944)
The ARHGEF18 Polyclonal Antibody (PAC031944) is a valuable tool for researchers studying ARHGEF18, a protein involved in cell signaling pathways and regulation of the cytoskeleton. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ARHGEF18 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for investigations in cell biology and cancer research.ARHGEF18, also known as a Rho guanine exchange factor, plays a crucial role in mediating cellular processes such as cell migration, adhesion, and proliferation.
Its involvement in signaling cascades makes it a key player in various cellular functions and suggests its potential significance in disease processes. Research on ARHGEF18 can provide valuable insights into cancer progression, metastasis, and other pathological conditions where dysregulation of cell signaling pathways is implicated.Overall, the ARHGEF18 Polyclonal Antibody offers researchers a reliable tool for studying the biology and functions of ARHGEF18 in health and disease. Its specificity and sensitivity make it a valuable asset for advancing our understanding of cellular signaling mechanisms and potential therapeutic targets in cancer and other diseases.
| Antibody Name: | ARHGEF18 Antibody (PACO31944) |
| Antibody SKU: | PACO31944 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Rho guanine nucleotide exchange factor 18 protein (159-551AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: ARHGEF18 antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 131, 115 kDa Observed band size: 30 kDa . |
| Background: | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RgoGEF cts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS), but does not act as a GEF for CDC42. The G protein beta-γ (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. |
| Synonyms: | Rho guanine nucleotide exchange factor 18 (114 kDa Rho-specific guanine nucleotide exchange factor) (p114-Rho-GEF) (p114RhoGEF) (Septin-associated RhoGEF) (SA-RhoGEF), ARHGEF18, KIAA0521 |
| UniProt Protein Function: | ARHGEF18: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. 2 isoforms of the human protein are produced by alternative splicing.Protein type: GEFs; Motility/polarity/chemotaxis; GEFs, Rac/RhoChromosomal Location of Human Ortholog: 19p13.3Cellular Component: apical part of cell; cell junction; cytoplasm; cytosolMolecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activityBiological Process: actin cytoskeleton organization and biogenesis; positive regulation of apoptosis; regulation of cell shape; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008] |
| UniProt Code: | Q6ZSZ5 |
| NCBI GenInfo Identifier: | 296439444 |
| NCBI Gene ID: | 23370 |
| NCBI Accession: | Q6ZSZ5.3 |
| UniProt Secondary Accession: | Q6ZSZ5,O60274, Q6DD92, A8MV62, B5ME81 |
| UniProt Related Accession: | Q6ZSZ5 |
| Molecular Weight: | 114,077 Da |
| NCBI Full Name: | Rho guanine nucleotide exchange factor 18 |
| NCBI Synonym Full Names: | Rho/Rac guanine nucleotide exchange factor 18 |
| NCBI Official Symbol: | ARHGEF18Â Â |
| NCBI Official Synonym Symbols: | P114-RhoGEFÂ Â |
| NCBI Protein Information: | rho guanine nucleotide exchange factor 18 |
| UniProt Protein Name: | Rho guanine nucleotide exchange factor 18 |
| UniProt Synonym Protein Names: | 114 kDa Rho-specific guanine nucleotide exchange factor; p114-Rho-GEF; p114RhoGEF; Septin-associated RhoGEF; SA-RhoGEF |
| Protein Family: | Rho guanine nucleotide exchange factor |
| UniProt Gene Name: | ARHGEF18Â Â |
| UniProt Entry Name: | ARHGI_HUMAN |
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